"Applications of Unbiased Stereology

to Neural Systems"

Established in1995, the SRC's training programs have educated hundreds of bioscientists in the principles and practices of modern, design-based (unbiased) stereology. Recognized experts in the fields of stereology and neuroscience deliver formal lectures, valuable insights, and practical experience through multimedia presentations, demonstrations of computerized stereology and group discussions, all in a relaxed, collegial atmosphere. 

• To familiarize participants with the theory and practice of modern stereology as applied to neural structure.
• To improve participants' skill in experimental design and critical analysis of quantitative morphometry of neural structure.
• To train participants on the use of cutting edge computerized stereology systems.
• To provide an informal environment for fruitful exchange of information between participants and instructors.

Dr. Peter R. Mouton, the official U.S. representative of the International Society For Stereology, organizes and teaches our one-day Stereology ShortCourses™ at a variety of locations in US, Canada, and abroad. At the Stereology Workshop™, Dr. Mouton is joined by several experienced stereologists and professional instructors, including:

• Professor Arun M. Gokhale, Georgia Institute of Technology, Atlanta, GA
• Jeffrey M. Long, Ph.D., University of Texas Southwestern Medical Center, Dallas, TX
• Kebreten F. Manaye, M.D., Associate Professor, Howard University College of Medicine, Washington, DC

These instructors know the art and craft of stereology. They understand the approaches, use them in their own research projects, and teach them for a wide variety of applications to biomedical research. Through years of experience they have learned to optimize the methods for optimal efficiency. Most importantly, they have a long-term dedication and commitment to participants who need to acquire these skills for their own research projects.

Principles and Practices of Unbiased Stereology: An Introduction for Bioscientists (2002) by Peter R. Mouton, Ph.D. is available at a 20% discount from the The Johns Hopkins University Press. For details, click here.

Computer-assisted Stereology

Our courses and workshop train participants in the latest methods of computer-assisted quantification of biological structure, techniques that enhance applications with all commercially available software packages.  Call or email us for further information on the best computerized approaches for your projects (scott@disector.com; 410.643.4901).

Sample of recent peer-reviewed studies using the Stereologer system:

Berman, R.F., Pessah, I.N., Mouton, P.R. Mav, D., Harry, G.J. Low Level Neonatal Thimerosal Exposure: Further Evaluation of Altered Neurotoxic Potential in SJL Mice. Toxicol. Sci.101(2):294-309, 2008.

O’Neil, J.N., Mouton, P.R., Tizabi Y., Ottinger, M.A., Lei, D-L., Ingram, D.K., Manaye, K.F. Catecholaminergic Neuron Number In Locus Coeruleus Of Aged Female Dtg APP/PS1 Mice. J. Chem.Neuroanat. 34(3-4):102-7, 2007.

Manaye, K.F., Wang, P., O’Neil, J., Huafu, S., Tizabi, Y., Thompson, N., Ottinger, M.A., Ingram, D.K., Mouton, P.R. Neuropathological Quantification Of Dtg APP/PS1: Neuroimaging, Stereology, And Biochemistry. AGE: 29:87-96, 2007.

Kimberly R. Byrnes, Bogdan A. Stoica, Stanley Fricke, Simone Di Giovanni2 and Alan I. Faden. Cell cycle activation contributes to post-mitotic cell death and secondary damage after spinal cord injury. Brain, August 9, 2007.

Perry, T.A., Holloway, H.W., Weerasuriya, A., Mouton, P.R., Duffy, K., Mattison J.A. Evidence of GLP-1-mediated neuroprotection in an animal model of pyridoxine-induced peripheral sensory neuropathy. Exp Neurol 203:293-301, 2007.

Duffy, K.B, Spangler E.L., Devan B.D., Guo Z., Bowker J.L., Janas, A.M., Hagepanos, A., Minor, R.K., DeCabo R., Mouton, P.R., Shukitt-Hale, B., Joseph, J.A., Ingram, D.K. A blueberry-enriched diet provides cellular protection against oxidative stress and reduces a kainate-induced learning impairment in rats. Neurobiol Aging. May 22, 2007

Vana, A, Flint, Nicole C, Harwood, NE, Le, TQ, Fruttiger, M, Armstrong, RC. Platelet-Derived Growth Factor Promotes Repair of Chronically Demyelinated White Matter. ournal of Neuropathology & Experimental Neurology. 66(11):975-988, November 2007.

S.H. Zanjani, F. Selimi, M.W. Vogel, A.M. Haeberle, J. Boeuf, J. Mariani, Y.J. Bailly
Survival of interneurons and parallel fiber synapses in a cerebellar cortex deprived of Purkinje cells: studies in the double mutant mouse Grid2Lc/+;Bax(-/-).J Comp Neurol. 2006, 497:622-35.

Francis HW, Rivas A, Lehar M, Saito Y, Mouton PR, Ryugo DK. Efficient Quantification Of Afferent Cochlear Ultrastructure Using Design-Based Stereology. J Neurosci Methods.150:150-8, 2006.

E. J. H. Schenck, C. L. Brooks. Effects of an S84E Mutation of Bovine Growth Hormone in Transgenic Mice. Experimental Biology and Medicine 2006, 231:296-302.

Pimonporn Chaovipoch, Karen A. Bozak Jelks, Lynnette M. Gerhold, Eric J. West, Sukumal Chongthammakun, Candace L. Floyd.
17β-Estradiol Is Protective in Spinal Cord Injury in Post- and Pre-Menopausal Rats.
J. Neurotrauma 2006, 23: 830-852.

Jianting Miao, Michael P. Vitek, Feng Xu, Mary Lou Previti, Judianne Davis, and William E. Van Nostrand
Reducing Cerebral Microvascular Amyloid- Protein Deposition Diminishes Regional Neuroinflammation in Vasculotropic Mutant Amyloid Precursor Protein Transgenic Mice.
J. Neurosci., 2005, 25:6271–6277.

Phinney AL, Calhoun ME, Woods AG, Deller T, Jucker M. Stereological analysis of the reorganization of the dentate gyrus following entorhinal cortex lesion in mice.
Eur J Neurosci. 2004 Apr;19(7):1731-40.

Daniel Goti, Scott M. Katzen, Jesse Mez, Noam Kurtis, Jennifer Kiluk, Lea Ben-Haïem, Nancy A. Jenkins, Neal G. Copeland, Akira Kakizuka, Alan H. Sharp, Christopher A. Ross, Peter R. Mouton, and Veronica Colomer
A Mutant Ataxin-3 Putative-Cleavage Fragment in Brains of Machado-Joseph Disease Patients and Transgenic Mice Is Cytotoxic above a Critical Concentration.
J. Neurosci. 2004, 24:10266–10279.

Sarah A. Baker, K. Adam Baker, Theo Hagg.
Dopaminergic nigrostriatal projections regulate neural precursor proliferation in the adult mouse subventricular zone.
European J Neurosci 2004, 20: 575–579.

W. Luo, Y. Chen, A. Liu, H.M. Lim, J. Marshall-Neff, J., Black, W. Baldwin, Hruban, R.H., S.C. Stevensen, P.R. Mouton. A. Dardik, B.F. Ballerman, Inhibition of Accelerated Graft Arteriosclerosis by GeneTransfer of Soluable FGF Receptor-1 in Rat Aortic Transplants.
Arterio Thrombosis Vascular Biology, 24:1081-6, 2004.

AM Gokhale, RE Evans, JL Mackes, PR Mouton. Stereological Estimation of Surface Area in Thick Transparent Sections of Arbitrary Orientation Using Virtual Cycloids.
J Microsc 216(Pt1): 25-31, 2004.

TA Perry, A Weerasuriya, PR Mouton, HW Holloway, NH Greig.
Pyridoxine-induced toxicity in rats: a stereological quantification of the sensory neuropathy.
Exp Neurol 190:133-44, 2004.

Kirkpatrick, Brian; Messias, Nidia C.; Conley, Robert R.; Roberts, Rosalinda C.
Interstitial Cells of the White Matter in the Dorsolateral Prefrontal Cortex in Deficit and Nondeficit Schizophrenia.
J Nervous & Mental Disease 2003, 191:563-567.

R.M. Sharpe, H.M. Fraser, M.F.H. Brougham, C. McKinnell, K.D. Morris, C.J.H. Kelnar, W.H.B. Wallace, M. Walker
Role of the neonatal period of pituitary–testicular activity in germ cell proliferation and differentiation in the primate testis.
Human Reproduction 2003, 18: 2110-2117.

D-L Lei, JM Long, J Hengemihle, DK Ingram, J O’Neill, KF Manaye, PR Mouton. Effects Of Estrogen And Raloxifene On Neuroglia Number and Morphology In The Hippocampus Of Aged Female Mice.
Neurosci 2003, 121:659-666.

AL Oaklander, EA Stocks, P.R. Mouton.
Number of Langerhans' immune cells in painful and non-painful human skin after shingles.
Archives Dermatology Research 294:529-535, 2003.

Regina C. Armstrong, Tuan Q. Le, Emma E. Frost, Rosemary C. Borke, and Adam C. Vana
Absence of Fibroblast Growth Factor 2 Promotes Oligodendroglial Repopulation of Demyelinated White Matter.
J. Neurosci., 2002, 22:8574–8585.

Sonia Boncristiano, Michael E. Calhoun, Peter H. Kelly, Michelle Pfeifer, Luca Bondolfi, Martina Stalder, Amie L. Phinney, Dorothee Abramowski, Christine Sturchler-Pierrat, Albert Enz, Bernd Sommer, Matthias Staufenbiel, and Mathias Jucker
Cholinergic Changes in the APP23 Transgenic Mouse Model of Cerebral Amyloidosis.
J. Neurosci., 2002, 22:3234–3243.

Luca Bondolfi, Michael Calhoun, Florian Ermini, H. Georg Kuhn, Karl-Heinz Wiederhold, Lary Walker, Matthias Staufenbiel, and Mathias Jucker
Amyloid-Associated Neuron Loss and Gliogenesis in the Neocortex of Amyloid Precursor Protein Transgenic Mice
J. Neurosci., 2002, 22:515–522.

Paul T. Jantzen, Karen E. Connor, Giovanni DiCarlo, Gary L. Wenk, John L. Wallace, Amyn M. Rojiani, Domenico Coppola, Dave Morgan, and Marcia N. Gordon
Microglial Activation and -Amyloid Deposit Reduction Caused by a Nitric Oxide-Releasing Nonsteroidal Anti-Inflammatory Drug in Amyloid Precursor Protein Plus Presenilin-1 Transgenic Mice.
J. Neurosci., 2002, 22:2246–2254.

C.J.H. Kelnar, C. McKinnell, M. Walker, K.D. Morris, W.H.B. Wallace, P.T.K. Saunders, H.M. Fraser, R.M. Sharpe
Testicular changes during infantile ‘quiescence’ in the marmoset and their gonadotrophin dependence: a model for investigating susceptibility of the prepubertal human testis to cancer therapy?
Human Reproduction, 2002, 17:1367-1378.

Inna I. Kruman, T. S. Kumaravel, Althaf Lohani, Ward A. Pedersen, Roy G. Cutler, Yuri Kruman, Norman Haughey, Jaewon Lee, Michele Evans, and Mark P. Mattson
Folic Acid Deficiency and Homocysteine Impair DNA Repair in Hippocampal Neurons and Sensitize Them to Amyloid Toxicity in Experimental Models of Alzheimer's Disease
J. Neurosci., 2002, 22:1752–1762.