"Good Stereology Through Good Engineering"

The STEREOLOGER system continues to lead the industry for state-of-the-art computerized stereology.

A generation ago, the international community of biomedical reseachers, including journal editors and grant reviewers, began to recognize design-based (unbiased) approaches of the STEREOLOGER as the state-of-the-art methods for the reliable and reproducible quantification of biological structure.

In the mid 1990s Dr. Peter R. Mouton teamed with Systems Planning and Analysis (Alexandria, VA) and the Johns Hopkins University School of Medicine (Baltimore, MD) to develop the STEREOLOGER system, the first integrated hardware-software stereology system for both PC and Macintosh computers.

During the next ten years, the STEREOLOGER system redefined the industry with a new standard for video microscopy integrated with innovative and cutting-edge unbiased stereology:

• State-of-the-art design-based stereology, the only system for both Macintosh and PC computers, all brands of microscopes, and all stage motors for movement in X-Y-Z axes.
• Top-selling computerized stereology system for the past five years.
• Price literally tens of thousands less than less advanced, less versatile, and less user-friendly systems.
• Only computerized stereology system designed with financial support and peer-review approval from the National Institutes of Health and the U.S. Public Health Service.
• Regularly reviewed and approved by professional stereologists and biomedical scientists.
• Only computerized stereology that fully incorporates virtual probes for analysis of tissue sections cut at any orientation (coronal, horizontal, etc.).
• On-site installation, training, and support available worldwide by professional stereologists and biomedical scientists.
• Includes free copy of the best-selling textbook for applications of design-based stereology to biomedical research, and the highly acclaimed system manual, "Introduction To Computerized Stereology."

The STEREOLOGER system allows you to test hypotheses in your research projects with maximal attention to the accuracy and precision of the results.

To request an online STEREOLOGER quote, contact Application Specialist, Scott McElhiney at:
Phone: 352-871-7045
Email: scott@disector.com.

Or click here to request an on-line quote.





State-Of-The-Art Resources For Quantitative Analysis Of Biological Tissue

In combination with a computerized stereology system, either the STEREOLOGER or any other system on the market, we recommend an SRC-approved training course and the best selling stereology text from the Johns Hopkins University Press. These stereology resources provide state-of-the-art training, important theoretical background, and practical details, the critical information required for effective, efficient, and affordable application of stereology principles to biological tissue.


Cost Of Stereologer Packages

At the SRC we promise to improve the productivity of your laboratory with a high-performance STEREOLOGER system at the lowest possible cost.

We can ship the STEREOLOGER software to your laboratory, preloaded on a new Mac or PC computer, for under $30K for the Basic Stereologer system and under $35K for the Advanced Stereologer system*,**. Just specify your microscope, add a monitor, and you're ready to go. Pure and simple.

*Differences in costs relate to cameras with differing resolution and other features.
** Prices include a new Macintosh or PC computer, a motorized XYZ stage and encoder, high resolution color camera, connection cables, stage micrometer, on-site installation, and annual maintenance agreement.

Let us know if you already own some or all of the required hardware (computer, motorized stage, camera). We will provide a quote to complete your system at a signficant savings off the full package price.

If you previously purchased an older version of the STEREOLOGER software, call us for a quote to upgrade your system to the current version 2.0, at a reduced cost of course.

Why spend more for state-of-the-art stereology than you have to?

To receive a quote for a Basic or Advanced system, to upgrade to the version 2.0, or to convert your current system to the STEREOLOGER, contact Scott McElhiney at:
Phone: 352-871-7045
Email: scott@disector.com.

Or click here to request an on-line quote.


Save your research dollars

Beware of vendors who quote significantly higher prices for computerized stereology systems, especially those that promise the ability to expand with "add-ons," which are obsolete features, equipment, and services that you do not need or will never use. Call us to discuss details about these add-ons, or compare the features from different vendors.

Cost-affordable Conversion To The STEREOLOGER System

Frustrated with your current stereology system? Unfortunately, vendors of other computerized stereology systems focus more efforts on gimmick "add-ons," agreesive sales tactics, and high powered marketing, rather than on the design and development of user-friendly systems. We often hear from unsatisfied scientists who have spent large amounts of money on these computerized stereology systems. At the Stereology Resource Center, this will never happen, because our customers come first, before sales, before marketing, before all other considerations.

When you are ready to trade up to the STEREOLOGER, call or email for a discounted quote to build a system that incorporates your existing hardware in the most optimal and cost-effective way. This conversion will bring the leading-edge of computerized stereology resources to your laboratory, and increase the productivity to your research program at the lowest possible cost.

Testimonials from STEREOLOGER users

“I enjoy using the STEREOLOGER system and most importantly, I trust the data. I find the system far better organized and far easier to use than the older approaches. Also, real scientists are available to answer questions about the STEREOLOGER. ”
Dr. D.L.L., Research Associate, user of the Mac-version at the Johns Hopkins University School of Medicine, Baltimore, MD.

"I've tried them all, including the StereoInvestigator and CAST systems, and prefer the STEREOLOGER."
Dr. D.G.O., neuroscientist, user of the PC-version in University of Toledo, Spain.

"The students and technicians in my laboratory have different computerized stereology systems to choose from, and they all prefer the STEREOLOGER."
Prof. R.B., behavioralist, user of the Mac-version University of California, Davis.

"...above all, we like the STEREOLOGER system. Congrats on the user-interface and technical support, all much more straightforward and easier-to-use than other systems."
Dr. G.T., postdoctoral scientist, user of the PC-version at the National Institute On Aging, NIH, Baltimore, MD.

Recent peer-reviewed publications using the STEREOLOGER

Perry T, Holloway HW, Weerasuriya A, Mouton PR, Duffy K, Mattison JA, Greig NH. Evidence of GLP-1-mediated neuroprotection in an animal model of pyridoxine-induced peripheral sensory neuropathy. Exp Neurol. Nov 21, 2006.

Armstrong, R.C., Le, T.Q., Flint, N.C., Vana,A.C., Zhou., Y-X. Endogenous Cell Repair of Chronic Demyelination. J Neuropathol Exp Neurol. March; 65(3): 245–256, 2006.

RJ Roper, LL Baxter, NG Saran, DK Klinedinst, PA Beachy, RH Reeves. Defective cerebellar response to mitogenic Hedgehog signaling in Down syndrome mice. Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1452-6.

E. J. H. Schenck, C. L. Brooks Effects of an S84E Mutation of Bovine Growth Hormone in Transgenic Mice. Experimental Biology and Medicine, 231:296-302, 2006.

E. Aberg; T. M. Pham, M. Zwart, V. Baumans, S.C.A. Brene. Intermittent individual housing increases survival of newly proliferated cells. Neuroreport. 16(13):1419-1422, 2005.

Pimonporn Chaovipoch, Karen A. Bozak Jelks, Lynnette M. Gerhold, Eric J. West, Sukumal Chongthammakun, Candace L. Floyd. 17β-Estradiol Is Protective in Spinal Cord Injury in Post- and Pre-Menopausal Rats. J. Neurotrauma 2006, 23: 830-852.

Jianting Miao, Michael P. Vitek, Feng Xu, Mary Lou Previti, Judianne Davis, and William E. Van Nostrand Reducing Cerebral Microvascular Amyloid- Protein Deposition Diminishes Regional Neuroinflammation in Vasculotropic Mutant Amyloid Precursor Protein Transgenic Mice. J. Neurosci., 2005, 25:6271–6277.

Daniel Goti, Scott M. Katzen, Jesse Mez, Noam Kurtis, Jennifer Kiluk, Lea Ben-Haïem, Nancy A. Jenkins, Neal G. Copeland, Akira Kakizuka, Alan H. Sharp, Christopher A. Ross, Peter R. Mouton, and Veronica Colomer A Mutant Ataxin-3 Putative-Cleavage Fragment in Brains of Machado-Joseph Disease Patients and Transgenic Mice Is Cytotoxic above a Critical Concentration. J. Neurosci. 2004, 24:10266–10279.

Sarah A. Baker, K. Adam Baker, Theo Hagg. Dopaminergic nigrostriatal projections regulate neural precursor proliferation in the adult mouse subventricular zone. European J Neurosci 2004, 20: 575–579.

Kirkpatrick, Brian; Messias, Nidia C.; Conley, Robert R.; Roberts, Rosalinda C. Interstitial Cells of the White Matter in the Dorsolateral Prefrontal Cortex in Deficit and Nondeficit Schizophrenia. J Nervous & Mental Disease 2003, 191:563-567.

R.M. Sharpe, H.M. Fraser, M.F.H. Brougham, C. McKinnell, K.D. Morris, C.J.H. Kelnar, W.H.B. Wallace, M. Walker Role of the neonatal period of pituitary–testicular activity in germ cell proliferation and differentiation in the primate testis. Human Reproduction 2003, 18: 2110-2117.

JA Olschowka, WJ Bowers, SD Hurley, MA Mastrangelo, HJ Federoff. Helper-free HSV-1 amplicons elicit a markedly less robust innate immune response in the CNS. Mol Ther. 2003 Feb;7(2):218-27.

Regina C. Armstrong, Tuan Q. Le, Emma E. Frost, Rosemary C. Borke, and Adam C. Vana. Absence of Fibroblast Growth Factor 2 Promotes Oligodendroglial Repopulation of Demyelinated White Matter. J. Neurosci., 2002, 22:8574–8585.

Sonia Boncristiano, Michael E. Calhoun, Peter H. Kelly, Michelle Pfeifer, Luca Bondolfi, Martina Stalder, Amie L. Phinney, Dorothee Abramowski, Christine Sturchler-Pierrat, Albert Enz, Bernd Sommer, Matthias Staufenbiel, and Mathias Jucker Cholinergic Changes in the APP23 Transgenic Mouse Model of Cerebral Amyloidosis. J. Neurosci., 2002, 22:3234–3243.

Luca Bondolfi, Michael Calhoun, Florian Ermini, H. Georg Kuhn, Karl-Heinz Wiederhold, Lary Walker, Matthias Staufenbiel, and Mathias Jucker Amyloid-Associated Neuron Loss and Gliogenesis in the Neocortex of Amyloid Precursor Protein Transgenic Mice J. Neurosci., 2002, 22:515–522.

Paul T. Jantzen, Karen E. Connor, Giovanni DiCarlo, Gary L. Wenk, John L. Wallace, Amyn M. Rojiani, Domenico Coppola, Dave Morgan, and Marcia N. Gordon Microglial Activation and -Amyloid Deposit Reduction Caused by a Nitric Oxide-Releasing Nonsteroidal Anti-Inflammatory Drug in Amyloid Precursor Protein Plus Presenilin-1 Transgenic Mice. J. Neurosci., 2002, 22:2246–2254.

C.J.H. Kelnar, C. McKinnell, M. Walker, K.D. Morris, W.H.B. Wallace, P.T.K. Saunders, H.M. Fraser, R.M. Sharpe  Testicular changes during infantile ‘quiescence’ in the marmoset and their gonadotrophin dependence: a model for investigating susceptibility of the prepubertal human testis to cancer therapy? Human Reproduction, 2002, 17:1367-1378.

Inna I. Kruman, T. S. Kumaravel, Althaf Lohani, Ward A. Pedersen, Roy G. Cutler, Yuri Kruman, Norman Haughey, Jaewon Lee, Michele Evans, and Mark P. Mattson Folic Acid Deficiency and Homocysteine Impair DNA Repair in Hippocampal Neurons and Sensitize Them to Amyloid Toxicity in Experimental Models of Alzheimer's DiseaseJ. Neurosci., 2002, 22:1752–1762.

Mouton PR, Gokhale AM, Ward NL, West MJ. Stereological Length Estimation Using Spherical Probes. J. Microscopy, 206: 54-64, 2002

Mouton, P.R., J.M. Long, E.A. Stocks, S. Rim, V. Howard, M. Jucker, M.E. Calhoun, D.K. Ingram. Age and Gender-based Differences in Astrocytes And Microglia in Hippocampal Subregions of C57BL/6J Mice. Brain Research 956:30-35, 2002.

Zanjani HS, , Vogel MW, Delhaye-Bouchaud N, Martinou JC, Mariani J. Increased cerebellar Purkinje cell numbers in mice overexpressing a human bcl-2 transgene. J Comp Neurol. 1996 Oct 21;374(3):332-41.

Govek EK, Wang J, Swann JM. Sex differences in the magnocellular subdivision of the medial preoptic nucleus in Syrian hamsters. Neuroscience. 2003;116(2):593-8.

Liu Z, Gastard M, Verina T, Bora S, Mouton PR, Koliatsos VE. Estrogens modulate experimentally induced apoptosis of granule cells in the adult hippocampus. J Comp Neurol 441:1-8, 2001.

Jucker M, Bondolfi L, Calhoun ME, Long JM, Ingram DK. Structural brain aging in inbred mice: potential for genetic linkage, Exp Gerontol 35:1383-1388, 2000.

Holtzman DM, Bales KR, Tenkova T, Fagan AM, Parsadanian M, Sartorius LJ, Mackey B, Olney J, McKeel D, Wozniak D, Paul SM. Apolipoprotein E isoform-dependent amyloid deposition and neuritic degeneration in a mouse model of Alzheimer's disease. Proc Natl Acad Sci U S A 14;97(6):2892-2897, 2000.

Farber NB, Rubin EH, Newcomer JW, Kinscherf DA, Miller JP, Morris JC, Olney JW, McKeel DW Jr. Increased neocortical neurofibrillary tangle density in subjects with Alzheimer disease and psychosis. Arch Gen Psychiatry 57:1165-1173, 2000.

Lee J, Duan W, Long JM, Ingram DK, Mattson MP. Dietary restriction increases the number of newly generated neural cells, and induces BDNF expression, in the dentate gyrus of rats. J Mol Neurosci 15(2): 99-108, 2000.

Calhoun ME, Mouton PR. New Developments In Neurostereology:Length Measurement And 3D Imagery. JChemNeuroanat1:61-9,2000.

Calhoun ME, Kurth D, Phinney AL, Long JM, Hengemihle J, Mouton PR, Ingram DK, Jucker M. Hippocampal neuron and synaptophysin-positive bouton number in aging C57BL/6 mice. Neurobiol Aging 1998 Nov; 19(6):599-606

Phinney AL, Calhoun ME, Wolfer DP, Lipp HP, Zheng H, Jucker M. No hippocampal neuron or synaptic bouton loss in learning-impaired aged beta-amyloid precursor protein-null mice. Neuroscience 90(4): 1207-1216, 1999.

Long JM, Mouton PR, Jucker M, Ingram DK: What Counts In Brain Aging? Design-Based Stereological Analysis Of Cell Number. J. Gerontology 54A: B407-B417, 1999.

Long JM, Mouton PR, Jucker M, Ingram DK. What counts in brain aging? Design-based stereological analysis of cell number. J Gerontol 54(10):B407-B417, 1999

Calhoun M.E., Wiederhold K.H., Abramowski D., Phinney A.L., Probst A., Sturcher-Pierrat C., Staufenbiel M., Sommer B., Jucker M. Neuron loss in APP transgenic mice. Nature, 395, 755-756, 1998.

Long, J.M., Kalehua A.N., Muth N.J., Hengemihle J.M., Jucker M., Calhoun M.E., Ingram D.K., and Mouton P.R. Stereological estimation oftotal microglia number in mouse hippocampus. J. Neuroscience Methods 1: 84:101-108, 1998.

Mouton PR, Martin LJ, Calhoun ME, Dal Forno G, Troncoso JC, Price DL. Cognitive Decline Strongly Correlates With Cortical Atrophy In Alzheimer’s Dementia. Neurobiol. Aging, 19: 371-377, 1998.

http://www.oregonlive.com/news/99/06/st061803.html

http://fizbin.eecs.lehigh.edu/~dahd/spec/